transcript%20density

Transcript density of Arachis hypogaea tissues mapped onto the combined A. duranensis and A. ipaensis genomes. Reads were provided by Peggy Ozias-Akins and sequenced by Brian Scheffler and Mary Duke. Samples were harvested and prepared by Ye Chu. Analysis by Josh Clevenger and Wei Huang. Sequences consisted of 2x100 Illumina-generated reads. Mapping was performed with TopHat v2.0.13, with options "no-discordant and no-mixed", and MAPQ score >=3 (up to two alignments allowed).

Tissues:

  • Lateral stem leaf :: Growth stage Boote R1: first flower; leaflets partially open, from laterals n+1
  • Mainstem leaf :: Growth stage Boote R1: first flower; leaflets partially open, from mainstem n
  • Seedling leaf :: 10 d post-emergence; leaflets partially open
  • Vegetative shoot tip :: Growth stage Boote R1: first flower, from mainstem n
  • Reproductive shoot tip :: Growth stage Boote R1: first flower, from laterals n+1
  • Root :: 10 d post-emergence
  • Nodule :: 25 d post-emergence
  • Perianth :: Petals, keel, hypanthium sepals No. 8a
  • Stamen :: Fully open, morning of anthesis; stamens
  • Pistil :: Fully open, morning of anthesis; pistils
  • Peg tip aerial :: From elongating peg prior to soil penetration
  • Peg tip below soil :: From elongating peg, 24 h after soil penetration
  • Peg basipetal to fruit Pattee 1 :: At pod swelling Pattee stage 1
  • Fruit Pattee 1 :: At pod swelling Pattee stage 1
  • Fruit Pattee 3 :: Pericarp very watery, embryo very small and not easily removed Pattee stage 3/4
  • Pericarp Pattee 5 :: Pericarp soft, not as watery, inner pericarp without cracks
  • Pericarp Pattee 6 :: Inner pericarp tissue beginning to show cracks or cottony
  • Seed Pattee 5 :: embryo flat, white or just turning pink at one end
  • Seed Pattee 6 :: Torpedo shaped; generally pink at embryonic-axis end of kernels
  • Seed Pattee 7 :: Torpedo shaped to round; embryonic end of kernel pink; other end white to light pink
  • Seed Pattee 8 :: Round, light pink all over
  • Seed Pattee 10 :: Large, generally dark pink all over; seed coat beginning to dry out

References:

  1. J. Clevenger, Ye Chu, Brian Scheffler and P. Ozias-Akins, A developmental transcriptome map for allotetraploid Arachis hypogaea. Front. Plant Sci. 7:1446, Pubmed.